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Instructions for Authors
 Electronic Submission Direct your manuscript to either of the two editors: Joseph H. Nadeau Genetics Department Case Western Reserve University 10900 Euclid Avenue Cleveland, OH 44106-4955 United States Email: jhn4@po.cwru.edu Steve D.M. Brown Medical Research Council Mammalian Genetics Unit Harwell, Didcot Oxfordshire 0X11 0RD United Kingdom Email: s.brown@har.mrc.ac.uk EDITORIAL POLICY Mammalian Genome publishes papers in all areas of genomic and genetic research with mice, humans, and other mammalian species. Areas covered include genetic and physical maps, genome organization and evolution, comparative gene mapping, complex trait analysis, new techniques, informatics as related to genome analysis, and human genetic disorders and their analysis within model organisms Mammalian Genome electronically processes all submitted manuscripts through the online center, Manuscript Central (http://mc.manuscriptcentral.com/mg). After your manuscript is submitted, the Editors will inspect the submission online and assign reviewers. PLEASE NOTE: For review purposes, please save and upload your TEXT (Title Page, Text, Acknowledgements, References, Tables, Legends) as one file. FIGURES may be uploaded as separate files. SYSTEM REQUIREMENTS Authors will need the following in order to use Manuscript Central:
AUTHOR ACCOUNTS Authors entering the journal's Manuscript Central site can either create a new account or use an existing one. When you have an existing account, use it for all your submissions and you can track their status on the same page. If you have submitted to Mammalian Genome before, please hit the "check for existing account" button. You will then receive an automatic e-mail with your user id and password. Otherwise please create a new account and then follow the instructions given on the screen. GETTING STARTED Once you have logged into your account, Manuscript Central will lead you through the submission process in a step-by-step orderly process. If you cannot finish your submission in one visit, you can save a draft and re-enter the process at the same point for that manuscript. While submitting your electronic manuscript, you will be required to enter data about your manuscript in the system. These include title, subtitle, author names and affiliations, and so forth. Support for special characters is available. At any point during this process, there are Help buttons available to see common questions and a support link to ask a specific question via email. UPLOADING FILES For review purposes, please save and upload your TEXT (Title Page, Text, Acknowledgements, References, Tables, Legends) as one file. FIGURES may be uploaded as separate files. Electronic files can be uploaded as PDF, PostScript, or RTF. PDF and PostScript files should already contain the graphics within the file. (PostScript files are converted by the system into PDF so that Editors and reviewers may share them. RTF (Rich Text Format) is a common export property of most popular word processors. Check your word processor to see if it can export or "SaveAs" your file in RTF format. MS Word and WordPerfect both contain this function. After uploading the RTF for text, you will be prompted for uploading graphics. Common graphics files such as GIF, JPEG, EPS, TIFF and many others are supported. After uploading the parts of the article in this manner, the system will convert the files to PDF. You will see the result of the conversion with the Acrobat plug-in in your browser. Keep copies of your word-processing and graphics files. You may want to revise the manuscript during the review process and you will need the original files if your manuscript is accepted. At any point during this process, there are Help buttons available to see common questions and a support link to ask a specific question via email. You will also be notified by email that your submission was successful. GRAPHICS QUALITY If you are submitting electronic graphics that you have scanned, be prepared to send the hard copy originals upon request. While the electronic files you have created are satisfactory for the review process, they may not be of sufficient quality for printing. This also holds true for files created in low-resolution graphics environments such as MS Powerpoint, etc. REQUIRED FORMS Certain forms may be required upon submission, or acceptance, of your manuscript. These forms can be found under the Icon, “Instructions and Forms” in the upper right-hand corner of the Manuscript Central screen. As soon as your manuscript has been submitted online, the “Copyright Form” must be printed out, signed and sent without delay to the Mammalian Genome Editorial Office at the following address: Louise Tinsley, Managing Editor Mammalian Genome MRC Mammalian Genetics Unit Harwell Didcot OX11 ORD Tel: +44 (0) 1235 841275 Fax: +44 (0) 1235 841200 E-mail: l.tinsley@har.mrc.ac.uk Other forms, such as the color reproduction form, may also be necessary if the manuscript is accepted for publication. If your manuscript is accepted, other hard copy items may also be requested later by the editors. KEEPING TRACK After submission, you may return periodically and monitor the progress of your submission through the review process. Manuscript Preparation Two types of manuscripts will be considered for publication:
Manuscripts are accepted for review with the understanding that they have not appeared in print or been submitted for publication elsewhere and that all authors have approved submission to Mammalian Genome. Articles and other materials published in the journal represent the opinions of the authors and do not necessarily reflect the views of the editors or publisher. Authors submitting a manuscript do so with the understanding that if accepted for publication, copyright (including the right to reproduce the article in all forms and media) shall be assigned exclusively to the publisher. The publishers will not refuse any reasonable request by authors for permission to reproduce their contribution. During electronic submission you will have the option to direct your manuscript for handling by either of the two editors: 5. Book, authored Blenkinsopp A, Paxton P. Symptoms in the pharmacy: a guide to the management of common illness. 3rd ed. Oxford: Blackwell Science; 1998. Manuscript Style and Format Manuscripts are submitted electronically through the journal web site for manuscript submission and review, http://mc.manuscriptcentral.com/mg and should be prepared using a word processing software package. The preferred platform is Microsoft Word, for either Macintosh or Windows operating systems. Manuscripts prepared with other word processors must be converted to Word format for uploading to the journal site. The text should be written using 2-line spacing, with about 1inch (2.5cm) margins, on 8.5 x 11 inch (letter size) or A4 page formats. Lettering should be 12 point (3 mm or 1/8 in). All pages should be numbered in sequential order starting with the title page. Include first names for all authors. Please follow the Guidelines for Electronically Produced Illustrations for preparation of figures. Manuscripts must be clearly and concisely written in English. They should be carefully scrutinized for errors before they are submitted. Correctness of spelling, grammar, and typing is the responsibility of the author. Foreign contributors, whose language is not English, should enlist the help of colleagues who are proficient in scientific English. Laboratory slang, clinical jargon, and colloquialisms are not acceptable. The Editors reserve the right to edit accepted manuscripts. Full-length manuscripts should be organized as follows: Title page, Abstract, Introduction, Materials and methods, Results, Discussion, Acknowledgments (including sources of support), References, Tables, and Figure legends. Please be sure to follow the journal style precisely in the composition of the manuscript. Manuscripts that do not conform to the conventions of the journal will be returned to the author for revision. Abbreviations and Nomenclature Avoid using unnecessary abbreviations and do not abbreviate names of organizations or institutions. The full term for which an abbreviation stands should precede its first use in the text unless it is a standard unit of measurement. Authors should use approved mouse nomenclature and this should be obtained prior to submission from Lois Maltais, The Jackson Laboratory, Bar Harbor, Maine 04609-0800, USA; tel: 207 288 6429; fax: 207 288 6132; e-mail: nomen@informatics.jax.org; http://www.informatics.jax.org/mgihome/nomen/index.shtml. Updated Mouse Nomenclature Guidelines can be found online at http://www.informatics.jax.org/mgihome/nomen/gene.shtml Chemical abstracts should be used as a guide for other abbreviations and symbols. Use S.I. units for linear dimensions, body mass, and clinical chemistry. Use the degree Celsius for temperature. Authors should use HUGO Gene Nomenclature Committee (HGNC) approved gene symbols for human genes (http://www.gene.ucl.ac.uk/nomenclature/). Symbols for human genes not found at the above website should be obtained prior to submission from the HUGO Gene Nomenclature Committee (HGNC), University College London, Wolfson House, 4 Stephenson Way, London NW1 2HE, UK; tel: +44 207 679 7410; fax:+44 207 387 3496; e-mail: nome@galton.ucl.ac.uk For guidelines see http://www.gene.ucl.ac.uk/nomenclature/guidelines.html and Wain et al.: Guidelines for human gene nomenclature. Genomics 2002; 79:464-470. Standard Abbreviations currently being used are: Chr Chromosome Da dalton kDa kilodalton L liter ml milliliter PCR polymerase chain reaction ORF open reading frame cM centimorgan bp base pair SDS sodium dodecylsulfate PAGE polyacrylamide gel electrophoresis FISH fluorescence in situ hybridization SSCP single strand conformational polymorphism LINE long interspersed element SINE short interspersed element MHC major histocompatibility complex HPLC high performance liquid chromatography SSLP simple sequence length polymorphism RFLP restriction fragment length polymorphism PFGE pulsed field gel electrophoresis Title page. The title page should be on a separate page and should include the complete title, the first name, middle name initial, and surname of all authors, as well as the institution of origin, including city, state, zip code, and country. The title must be limited to 85 characters, including spaces. For multi-authored papers, correspondence and page proofs will be sent to the first author, unless the editors are instructed otherwise. The title page should also include a Fax number, a voice phone number, and an E-mail address if one is available. A short title for use as a running head (not to exceed 55 characters, including spaces) should be provided. Abstract. Abstract must not exceed 250 words and should state the purpose of the study or investigation, basic procedures, main findings, and principal conclusions. Emphasize new and important aspects of the study or observations. It should be intelligible to scientists in other specialties and generally understood without access to the entire document. Avoid using references in the Abstract. Introduction. State briefly the nature and purpose of the article and refer to any relevant literature, concentrating on any recent important work done by others. The repetition of information in the Introduction and Discussion sections is undesirable. Materials and methods. Include only those technical details that are novel to the work presented in the manuscript. Provide citations only for techniques and protocols essentially equivalent to those published elsewhere. Results and discussion. Explain your results, and define their significance. Unsubstantiated speculation and plans for future study are not appropriate. References. Citations in the text should be by author and year. If there are only two authors, both should be listed. If there are more than two authors, only the first author's surname and "et al." are needed. Only references cited in the text and accepted for publication should be listed in alphabetical order by first author's surname in the reference section. Please double-space the reference section. Journal abbreviations listed in Index Medicus should be used. Unpublished data should be referred to parenthetically in the text with the author's name and "unpublished." Information provided by a colleague who is not an author should be indicated with the colleague's name followed by "personal communication." A letter from the colleague indicating permission to use the information must be submitted with the manuscript. Examples of reference format are the following: Lyon MF (1984) Transmission ratio distortion in mouse t-haplotypes is due to multiple distorter genes acting on a responder locus. Cell 37, 621-628 Sambrook J, Fritsch EF, Maniatis, T (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press) Arnheim N (1983) Concerted evolution of multigene families. In Evolution of Genes and Proteins, M. Nei and R.K. Koehn, eds. (Sunderland, Mass.: Sinauer Associates), pp 38-61 Tables. Tables should be numbered consecutively with arabic numerals in order of appearance in the text and should be typed double-spaced on separate pages. They should be collected together in numerical order after the references. Table titles should be clear, concise, and self-explanatory. A short descriptive caption should follow the title. Footnotes, which should be indicated by superscript lowercase letters, should be typed directly below the table. Complex tables may be submitted as camera-ready copy. These tables should be single-spaced and submitted as glossy prints, as well as conform to the journal's table format. Figures and illustrations. These should be numbered consecutively with arabic numerals in order of appearance in the text. Figures with biological information should be submitted as unmounted glossy prints. Computer-generated line drawings can be submitted as laser printer output on clean white paper. Each print should be identified on the reverse with the first author's last name and figure number and with the top of the figure or illustration marked. Figures should be designed to fit into one column (3.25 in or 8.5 cm wide) or two (7 in or 8.5 cm) with the final size of all lettering no larger than 12 point (3 mm or 1/8 in). Captions should be typed double-spaced on a separate page. The total number of illustrations may not exceed one less than half of the number of text pages. The additional cost of reproducing or printing large quantities of figures may be charged to the author. Illustrations that do not abide by these rules and those that do not make efficient use of space will be returned to authors for re-drawing. This process could hold up publication of accepted manuscripts. Color figures will be printed at the author's expense: $1,150.00 per article. Equations. Equations should be typewritten and the equation number, if necessary, placed in square brackets at the right margin. Reference to a specific equation should be written "Eq [1]." Footnotes. Footnotes should be used sparingly, numbered consecutively, and presented separately at the end of the manuscript. Reviewing All submitted manuscripts deemed appropriate for consideration by the editors will be peer reviewed. Those not considered appropriate will be returned to the authors immediately. Decisions on all papers peer-reviewed are normally sent to authors by four (4) weeks after submission. Proofs Page proofs will be sent to the corresponding author. Corrected proofs must be returned within 48 hours. Authors will be charged for extensive alterations. Reprints Reprints may be ordered at cost by using the form included with proofs. Distribution of genetic materials Publication of an article in Mammalian Genome is taken to indicate that the authors are prepared to freely distribute to academic researchers for their own use any cell lines, DNA, antibodies, mice, or other unique genetic materials used in the experiments reported. Failure to comply with this policy may result in exclusion from publication in Mammalian Genome. Submission of information to genetic databases. Authors must provide relevant information to the appropriate public databases before a manuscript can be accepted for publication. Information and addresses for the following databases are available from the Editorial Office: Fundamental Genetic Databases for the Mouse (GBASE, HMDP, and MLDP), GenBank and EMBL, Genome Database (GDB), and LODSOURCE (lod score for pairs of genetic markers, including disease loci and DNA polymorphisms). Accession numbers or copies of data-entry forms must be submitted to the editors as proof of compliance. New nucleotide date must be submitted and deposited in the DDBJ/EMBL/GenBank databases and an accession number obtained before the paper can be accepted for publication. Submission to any one of the three collaborating databanks is sufficient to ensure data entry in all. The accession number should be included in the manuscript as a footnote on the title page: “Note: Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession number(s)………”. If requested the database will withhold release of data until publication. The most convenient method for submitting sequence is by World Wide Web: EMBL via Webin: http://www.ebi.ac.uk/embl/Submission/webin.html GenBank via Bankit: http://www.ncbi.nlm.nih.gov/BankIt/ DDBJ via Sakura: http://sakura.ddbj.nig.ac.jp/ Alternatively, the stand-alone submission tool ‘Sequin’ is available from the EBI at http://www.ebi.ac.uk/Services/Sequin and from NCBI at http://www.ncbi.nlm.nih.gov/Sequin/ For special types of submissions (e.g. genomes, bulk submissions etc) additional submission systems are available from the above sites. Contact Information: EMBL: EMBL Nucleotide Sequence Submissions, European Bioninformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD UK; tel +44 1223 494400; fax: +44 1223 494472; e-mail: datasubs@ebi.ac.uk; http://www.ebi.ac.uk. GenBank: National Center for Biotechnology Information, National Library of Medicine, Bldg. 38A, Rm 8N-803, Bethesda, MD 20894, USA., tel: 301 496 2475; fax: 301 480 9241. Protein sequences, which have been determined by direct sequencing of the protein, must be submitted to SWISS-PROT at the EMBL Outstation—The European Bioinformatics Institute. Please note that we do note provide accession numbers, IN ADVANCE, for protein sequences that are the result of translation of nucleic acid sequences. These translations will automatically by forwarded to us from the EBML nucleotide database and are assigned SWISS-PROT accession numbers on incorporation into TrEMBL. Results from characterization experiments should also be submitted to SWISS-PROT at the EBI. This can include such information as function, subcellular location, subunit etc. Contact Information: SWISS-PROT submissions, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SD, UK; tel +44 1223 494400; fax: +44 1223 494472; e-mail: datasubs@ebi.ac.uk (for sequence submissions); update@ebi.ac.uk (for characterization information); http//www.ebi.ac.uk The HUGO Gene Nomenclature Committee (HGNC) is responsible for approving and implementing unique human gene symbols and names, and works closely with the Mouse Genome Database and other organism databases. Considerable efforts are made to approve symbols acceptable to workers in the field, but sometimes it is not possible to use exactly what has previously appeared in the literature. In such cases the previously used symbols are listed as aliases for the approved nomenclature in the HGNC Database http://www.gene.ucl.ac.uk/cgi-bin/nomenclature/searchgenes.pl, and other databases including Entrez Gene, to allow retrieval of all the information available for each gene. Copyright Submission of a manuscript implies that the work has not been previously published, nor is it under consideration for publication elsewhere. Also, any coauthors must approve the article for publication; if the manuscript is accepted for publication in Mammalian Genome, the author(s) agree to automatic transfer of the copyright to the publisher. Guidelines for Electronically Produced Illustrations for Print GENERAL
VECTOR (line) GRAPHICS
SPREADSHEET/PRESENTATION GRAPHICS
HALFTONE ILLUSTRATIONS
SCANS*
* We do prefer having the original art as our printers have drum scanners which allow for better reproduction of critical medical halftones. GRAPHICS FROM VIDEOS
Guidelines for Electronically Produced Illustrations for ONLINE VIDEO
Appendices and Supplementary Material Authors who wish to publish electronic supplementary material to their article (Excel files, images, audio/video files) are requested to submit their manuscript via our online submission system, with a note in the author comments box that supplementary material will be submitted separately by email. This email, containing the supplementary material, should be sent to the Production Editor. In the email, the title of the submitted manuscript as well as the name of the corresponding author should be given.
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